It is particularly important for social development to guide college students to advocate positive public opinion and oppose negative public opinion in the network space. Since network literacy is an important factor to measure college students’ quality education, it is urgent to explore a practical and effective training paradigm. Based on the in-depth analysis of the connotation of memetics and the status que of teaching college students’ network literacy, we carried out a teaching design of college students’ network literacy including the four stages of the life cycle of memetics. Theoretical teaching corresponds to the stage of meme assimilation and meme memory, improving students’ comprehensive knowledge of network literacy. Practical teaching corresponds to the expression of memes, improving their skill in network literacy. Teaching tracking corresponds to the transmission of memes, improving their network literacy at the psychological level. This research can provide new ideas for college students’ network literacy education and teaching.