Since the current “Makerspace” lacks a relatively mature operating mechanism, academic research at home and abroad has made great progress in clarifying the connotation, development elements and mechanism of Makerspace in recent years in spite of inadequate theoretical research on the operation mechanism of Makerspace. Based on the logic of “goal guidance-principle guarantee-mechanism construction”, this essay clarifies the principles of constructing the operation mechanism of Makerspace by analyzing its construction objectives. Then a set of three-layer and three-dimensional operation mechanism of Makerspace is constructed so as to enlighten the development of Makerspace in China.