Presence and mass are two important concepts proposed by Martin and other scholars based on Maton’s Legitimation Code Theory. The two terms are counterparts of Maton’s concepts of semantic gravity and semantic density. Presence and mass are key parameters of knowledge construction and are ideationally realized by iconicity and technicality respectively which are closely related to the frequencies of grammatical metaphors and technical terms. This paper explores the knowledge structural features of the advanced business English courses from the perspective of presence and mass with a case study of An Integrated Course of Advanced Business English. It shows that advanced business English courses belong to horizontal knowledge structure, are generally direct featuring with high presence value and low mass value, and are with low frequencies of the power grammar and power word. The results prove that the knowledge structure of advanced business English courses is designed in accordance with the curriculum standards for business English major. This study has important implications for compilation of business English textbooks and business English course teaching.